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KMID : 0880220100480060881
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Journal of Microbiology
2010 Volume.48 No. 6 p.881 ~ p.884
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Initial acidic pH is critical for mycelial cultures and functional exopolysaccharide production of an edible mushroom, Laetiporus sulphureus var. miniatus JM 27
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Seo Min-Jeong
Jeong Yong-Kee
Kim Min-Jeong
Lee Hye-Hyeon
Kim Sung-Ryeal
Kang Byoung-Won
Park Jeong-Uck
Rhu En-Ju
Choi Yung-Hyun
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Abstract
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We conducted a time course experiment on mycelial cultures of Laetiporus sulphureus var. miniatus. The strain showed significant survival in an initial pH range of 2.0 to 7.0 for 24 days, during which time oxalic acid was accumulated. A structural analysis of purified exopolysaccharide suggested that it contained 96.1% glucose, and the mode of linkage was mainly→4-Glcp-(1→units, with branches at the C-6 position consisting of a Glcp-(1→4) linked side chain. An exopolysaccharide purified from the acidophilic strain was added to cultured U937 cells, resulting in significantly increased transcription levels of p53 and p21 genes.
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KEYWORD
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mushroom, Laetiporus sulphureus var. miniatus, oxalic acid, p53, U937
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